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Questions related from Shuaichen Liu
Hi researchers, As the title shows, recently I made a construct with a 8xHis tag at N ternimal of HBx (154 aa). The voctor is pCDNA3.1+ and the sequencing result showed everything was correct. I...
08 November 2017 1,691 4 View
Hi researchers, I added a 8x His tag to my studying protein and I'm going to over express it then pull down it and detect it's interact partner. I'm looking for a column product instead of beads....
19 October 2017 2,123 6 View
Hi there, I did a de novo structure prediction for a protein (A) and got the PDB file for A. A has another genotype (B). Now I want to predict the structure of B, using A as a template, any...
23 August 2017 2,603 9 View
Hi all, I'm going to do immunoprecipitation using lifetech Dynabeads Protein G. I know for this experiment, I can incubate antibody and beads first then add to lysate or incubate antibody and...
22 July 2017 6,165 1 View
Hi there, I want to treat several liver cell lines with IL-6 and then check some genes' expression at mRNA level using real time PCR. Is there any recommendation for positive control in this...
03 July 2017 7,040 4 View
Hi researchers, I'm totally new to cell synchronization experiment. I want to arrest HepG2 cell at M phase and check the expression of a cell cycle related protein. I searched for some prorocols....
08 June 2017 7,607 7 View
Hi researchers, I'm searching the answer at the same time when I put the question. Appreciate any experience for this question. 1.For a know transcription factor (TF), how to check its binding...
08 May 2017 8,771 3 View
Hi dear researchers, I'm checking a table for a antibody array result. For each protein the company detect, they give a Swiss prot ID which can be checked at http://www.uniprot.org/. I want to ask...
18 April 2017 6,012 2 View
Hi researchers who study BMP2, I'm doing western blot for BMP2 recently and really need some help from those who ever done western for this protein! My major question is what is the real size for...
18 January 2017 6,666 3 View
Hi researchers, I made a construct with a Flag tagged to the N-terminal of my aim protein. I've already done the lentivirus infection and selected for over 2 weeks. I did a real time PCR to check...
03 January 2017 3,775 7 View
Hi researchers, I try to find a pair of primers for CXCL12 from publications. One strange thing I found was that, we I use the primers from publication to do blast in NCBI primer tool, they cannot...
05 September 2016 2,993 3 View
Hi researchers, I want to look at the mRNA expression data for a certain cell line (if someone has done it) in public database, how can I do that? For example, if I want to check HepG2.2.15 cell...
17 August 2016 8,914 4 View
Hi researchers, I want to see whether chronic hepatitis B infection will increase the expression of one gene. I'm not sure how to “generate” this condition. Can I use just inflammation cytokines...
27 July 2016 2,751 1 View
Hi researchers, I want to ask a question about ELISA dilution factor with my data. For the same sample, the first time I used 1:2 dilution (1 plasma + 1 diluent) and the OD value was 2.482. The...
25 July 2016 9,686 4 View
Hi researchers, Now I'm sequencing a 8kb PCR product from patient sample. I'm doing gel purification after PCR to get my product. One difficulty in this procedure is that I need to do several...
20 May 2016 9,941 4 View
Hi researchers, I'm using Incucyte to do proliferation assay for HCT116. I seeded 3000 cells/well in 96-well plate. I've done this several times and the cells grew ok. But this time, the cell...
20 April 2016 4,650 15 View
Hi researchers, My colleague passed me same serum samples (300ul each tube). He thawed it from stock and his operation was normative. I wanted to aliquot 50ul from each tube but I didn't know I...
18 April 2016 970 17 View
Hi dear researchers, Now I'm using the MinION to sequence a long PCR product. I realize that maybe during the end repair process, I lost a lot of my DNA sample, because there is an obvious...
24 March 2016 6,321 15 View
When I'm designing primers using NCBI primer tool, the explanation for Tm value is "The Tm calculation is controlled by Table of thermodynamic parameters and Salt correction formula (under...
01 February 2016 6,604 6 View
I'm trying to PCR from genomic DNA. After designing the primers, I blast them using NCBI blast tool. But I can hardly find suitable primers because they all have other binding sites in the genome....
27 January 2016 4,844 14 View
As the title, you input the amino acid sequence and then get the predicted 3D protein structure.
01 January 1970 3,756 7 View
Is there any method can detect the length of free DNA fragments in cytoplasm？ Thank for reply!
01 January 1970 7,860 0 View
Hi all, I'm not sure about this question. By prediction, I know there are several NF-kB binding sites among the promoter of my interested gene, but how I know which which dimmer are they? (p65,...
01 January 1970 2,509 5 View
I searched PubMed and Google and only found one similar to my interest. If anyone had read such kind of review and can recommend to me? Thanks!
01 January 1970 1,788 0 View
