Shreyasri Dutta

6 Questions 8 Answers 0 Followers

Questions related from Shreyasri Dutta

Trouble shooting of colchicine solution?

In my Karyotyping process, I used two falcon tubes with 3.75 ml RPMI and 1.2ml FBS with antibiotic for cell culturing purpose. Add bone marrow or Peripheral blood as per counting 75 lakh per...

20 March 2025 8,244 1 View

Can anyone share procedure of qualitative BCR-ABL test from EDTA blood?

Quantitative BCR-ABL done on routine basis, but want to test qualitatively. So any one please help me.

09 November 2022 1,405 0 View

For Galactomannan ELISA biorad kit, my cutoff and negative control are out of range, how can I over come this problem?

In my lab, I am using Biorad Galactomannan ELISA kit. During ELISA process, I washed the plate manually and using Ebra LisaScan reader for getting absorbance at 450/630 nm. But during validation...

27 July 2022 9,205 6 View

Which instruments are must for quantitative test of EGFR, BRAFV600E, KRAS, BRCA 1&2, PIK3CA, BCR-ABL(Ma, Mi, micro), PML RARA, DPD mutation)?

qPCR, capillary sequencer

15 February 2022 3,019 5 View

Can anyone suggest me whcih PCR method gives best sensitivity and specificity of EGFR test?

ARMS PCR or End point PCR or CAST PCR which is gives the best result

29 January 2022 9,520 6 View

Should DNA and RNA extraction done in separate BIOSAFETY CABINET or done simultaneously by UV on for 15 to 20 mins?

basic question of Molecular pathology

01 January 1970 4,320 2 View