Shirley Lin

4 Questions 2 Answers 0 Followers

Questions related from Shirley Lin

What is the function of Magnesium ions in immuno-precipitation buffer?

I noticed that almost all of the IP buffer containing magnesium ions, but I don't know the exact function of Mg2+ in the cell lysis process. Could anyone provide the answer of it? To my...

08 June 2018 6,116 3 View

How entecavir works in hepatitis B?

As we know, entecavir is a DNA analogue that can inhibit HBV reverse transcription, by integrating itself into HBV genome during viral replication and blocking the cccDNA formation due to lack of...

29 October 2017 8,361 1 View

Have anyone used nigericin sodium salt before? Why there is precipitation in cell medium when I use it?

I'm using nigericin to stimulate NLRP3 inflammasome in THP1 cell recently. The nigericin is diluted to 100mM with ethonal and preserved in -20 degree. The experiment protocol is: 1. Treated THP-1...

10 October 2016 2,333 2 View

The overexpressed protein can be detected in IFA but cann't be detected by WB?

I recently fused two ORF protein with IgG Fc fragment in pCAGEN vector. After transfection in 293T cell, using two mAbs of the ORF or the antibody against human IgG, I can observe obvious...

09 June 2016 5,510 2 View