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Questions related from Shirley Lin
I noticed that almost all of the IP buffer containing magnesium ions, but I don't know the exact function of Mg2+ in the cell lysis process. Could anyone provide the answer of it? To my...
08 June 2018 6,148 3 View
I recently did Co-IP between over-expressed protein, A and B, in 293T cells. At 36hpt, there is obvious interaction between the two proteins, while when I did immunofluoresence staining in HeLa...
03 May 2018 9,596 4 View
As we know, entecavir is a DNA analogue that can inhibit HBV reverse transcription, by integrating itself into HBV genome during viral replication and blocking the cccDNA formation due to lack of...
29 October 2017 8,387 1 View
I recently used 10 microliter/ml LPS to stimulate Hela cell, but didn't detect any IL-1B by Western Blot in cell lysates at 28h, did anyone use it before?
07 December 2016 1,764 1 View
I'm using nigericin to stimulate NLRP3 inflammasome in THP1 cell recently. The nigericin is diluted to 100mM with ethonal and preserved in -20 degree. The experiment protocol is: 1. Treated THP-1...
10 October 2016 2,386 2 View
I recently fused two ORF protein with IgG Fc fragment in pCAGEN vector. After transfection in 293T cell, using two mAbs of the ORF or the antibody against human IgG, I can observe obvious...
09 June 2016 5,561 2 View
