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Questions related from Sherif Arafa
I have a sample dissolved in DMSO.. Can i run this sample through Q sepharose column ?
12 March 2021 8,725 1 View
I want to do a cell based assay. This assay contains 5 uL of my cells (Dissolved in DMEM medium) and 5 uL of compound (dissolved in DMED medium also). Suppose I count the cells in my suspension...
20 October 2020 2,613 1 View
I am trying to do an enzyme assay using Wild type enzyme the protocol involves tris buffer pH 8, MgCl2, S-adenosyl methionine, Substrate, and I inititate the reaction with my enzyme, but after...
19 July 2019 2,325 3 View
I am trying to express mutants of my protein whose size is 30 Kda... however following purification by Ni affinity column ... the SDS PAGE shows the mutants appearnig at size 20 Kda.. is there a...
07 May 2019 6,093 14 View
Do I need to add chloramphenicol to LB agar media when trying to transform my plasmid into BL21 pLyss or I can add only the antibiotic of my plasmid?
01 May 2019 3,609 4 View
If I have a protein I want to crystallize and I tried several screening kits having different conditions but no crystals appear.. usually what is the next thing to do ? or it is better to give up...
01 March 2019 3,550 3 View
Now I am elucidating a crystal structure of a protein.. and my last step was fitting my ligand (FAD) into my protein. I knew that I have to calculate something omit map .. but I don't understand...
05 December 2018 1,069 2 View
II am currently working on a protien to be crystallized. The protien is bound to FAD, that's why when I purify the protein with Ni affinity column its colour was yellow . but surprisingly, after...
02 August 2018 2,999 3 View
I am trying to express and purify a protein after doing all the steps and purify using Ni affinity column but after doing the SDS PAGE I found that the amount of protein in the elute is very...
09 June 2018 2,020 5 View
I am trying to purify a protein..First I run Ni affinity column and the protein was eluted with KPB pH7.9, imidazole 500mM and glycerol ..then I diluted with No Salt buffer containg 50mM TrisHCL...
29 May 2018 6,442 7 View
If i have an aromatic ring with methoxy gp on it and 3 aromatic protons in ortho, meta and para position ..Is there an explanation for why the ortho proton appears at lower chemical shift than...
06 October 2017 10,055 4 View
I have cultured soil on 2 medium .. humic acid medium and chitin agar medium for culture of fungi and actinomycetes.. the question is .. how can i know the colon grown is fungi or bacteria .....
04 September 2017 3,237 6 View
I have a fraction that shows 2 spots on reversed phase TLC with solvent system methanol : water 1:1 .. now i want to purify this fraction but the problem is that its weight is 5 mg and i know that...
25 May 2017 5,917 7 View
What is the best mobile phase for isolation of compounds from Aloe vera flower extract?
06 January 2016 3,218 2 View
