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Questions related from Sharif Moradi
Hello guys, Recently I came across a comment mentioning that MgCl2 addition could enhance the efficiency and quality of RNA isolation. I asked the person who made the comment but did not get any...
02 February 2018 9,833 7 View
Hi there, I know that there are very good kits for miRNA isolation, but I want to use Trizol for this purpose. It is known that Trizol selectively loses GC-rich small RNAs when extracting RNA...
12 December 2017 5,206 12 View
Hi there, It has been a long time that I think about one of the important aspects of miRNA research: how many genes mediate miRNA effects? According to the literature, most if not all of miRNAs...
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Does anyone of you know how many cells is needed to run a cell cycle distribution analysis using flow cytometry (PI staining)? Mouse ES cells have been treated with a certain agent and I expect...
01 January 2015 1,767 15 View
I am going to profile microRNAs by small RNA sequencing. The starting material that is going to be used for microRNA isolation is too small. So we decided to isolate small RNAs by three different...
09 September 2014 9,168 9 View
I am going to order chemically synthesized ds-microRNAs. Which do you recommend? Should I order a perfectly matched ds RNA sequence, or a partially matched dsRNA identical to the mature microRNA...
04 April 2014 6,725 1 View