Sharif Moradi

6 Questions 19 Answers 0 Followers

Questions related from Sharif Moradi

Function of MgCl2 in RNA isolation?

Hello guys, Recently I came across a comment mentioning that MgCl2 addition could enhance the efficiency and quality of RNA isolation. I asked the person who made the comment but did not get any...

02 February 2018 9,825 7 View

Trizol for small RNA extraction?

Hi there, I know that there are very good kits for miRNA isolation, but I want to use Trizol for this purpose. It is known that Trizol selectively loses GC-rich small RNAs when extracting RNA...

12 December 2017 5,204 12 View

How many mRNA targets mediate the effects of a single miRNA? A single target?

Hi there, It has been a long time that I think about one of the important aspects of miRNA research: how many genes mediate miRNA effects? According to the literature, most if not all of miRNAs...

08 August 2017 892 7 View

What is the minimal cell number per sample for cell cycle profiling?

Does anyone of you know how many cells is needed to run a cell cycle distribution analysis using flow cytometry (PI staining)? Mouse ES cells have been treated with a certain agent and I expect...

01 January 2015 1,764 15 View

How can I do amplification of mature microRNA isolated from a very small amount of starting material for small RNA sequencing?

I am going to profile microRNAs by small RNA sequencing. The starting material that is going to be used for microRNA isolation is too small. So we decided to isolate small RNAs by three different...

09 September 2014 9,165 9 View

Any recommendations on ordering synthetic mature double-stranded (ds) microRNAs?

I am going to order chemically synthesized ds-microRNAs. Which do you recommend? Should I order a perfectly matched ds RNA sequence, or a partially matched dsRNA identical to the mature microRNA...

04 April 2014 6,724 1 View