4 Questions 5 Answers 0 Followers
Questions related from Shanti Pal Gangwar
Hi all, I have purified a protein from E. coli based system. All the purification steps were done without any ligand. However, the crystal structure of the protein showed a ligand density in the...
08 February 2021 8,629 5 View
Hi I want to create stable cell lines for membrane proteins of interest in inducible expression system. I wonder if anyone has used pTRE-tight-BI system to express two genes simultaneously in...
13 March 2020 2,930 3 View
I am trying to purify a protein of interest from E coli periplasm. The protein is tagged with pelB signal peptide at N term and expressed very well. I tried to purify it using osmotic shock...
17 September 2019 6,651 4 View
Hello All, I am doing a protein-peptide thermal shift assay in QuantStudio3. I have observed that most of the peptides are giving a significant negative shift in Tm, though some are giving a...
20 August 2018 1,411 4 View
