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Hi all, I have purified a protein from E. coli based system. All the purification steps were done without any ligand. However, the crystal structure of the protein showed a ligand density in the...
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Hi I want to create stable cell lines for membrane proteins of interest in inducible expression system. I wonder if anyone has used pTRE-tight-BI system to express two genes simultaneously in...
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Hello All, I am doing a protein-peptide thermal shift assay in QuantStudio3. I have observed that most of the peptides are giving a significant negative shift in Tm, though some are giving a...
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