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Questions related from Shanti Pal Gangwar
Hi all, I have purified a protein from E. coli based system. All the purification steps were done without any ligand. However, the crystal structure of the protein showed a ligand density in the...
08 February 2021 8,679 5 View
Hi I want to create stable cell lines for membrane proteins of interest in inducible expression system. I wonder if anyone has used pTRE-tight-BI system to express two genes simultaneously in...
13 March 2020 3,025 3 View
I am trying to purify a protein of interest from E coli periplasm. The protein is tagged with pelB signal peptide at N term and expressed very well. I tried to purify it using osmotic shock...
17 September 2019 6,706 4 View
Hello All, I am doing a protein-peptide thermal shift assay in QuantStudio3. I have observed that most of the peptides are giving a significant negative shift in Tm, though some are giving a...
20 August 2018 1,504 4 View
I am screening a protein-peptides interaction in thermal shift assay. I observe that few peptides are giving rise in ΔTm > 2.0 °C which is considered as potential hit as they seem to stabilize...
28 September 2017 2,155 1 View
I am studying protein-peptide interaction using fluorescence polarization. The peptides are TMR tagged at N-terminal. When I incubate a range of unlabeled protein concentration (0-2000nM) to the...
25 March 2017 1,121 6 View
I am studying the Protein-peptides interaction using FP assay. But my curve in never saturating even when unlabeled protein concentration is 1 mM. When I increase conc of unlabeled protein from...
16 September 2016 8,942 2 View
