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Buffer Solution contains Tris-HCl (pH 8.0), KCl, MgCl2, DTT, and EDTA. This problem has occurred during 5 consecutive iterations. The buffer itself is clear and the glycerol itself is clear, but...
11 December 2015 4,124 4 View
Hi, I am looking to calculate the Osmolarity of the following solution. Any help is most appreciated! 4uL H20 480uL 500mM Potassium Glutamate 400uL 50% Glycerol 80uL 1M HEPES 3uL 1M MgCl2 1uL 1M...
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I am purifying a DNA-binding protein for use in in vitro transcription assays. However, solubility has been a huge issue. I have made a C-terminal fusion to MBP, and get great expression and can...
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