Shabbir khan

10 Questions 14 Answers 0 Followers

Questions related from Shabbir khan

Does astrocyte morphology change in reactive states?

Hi everyone, I have some questions regarding astrocytes morphology, primary culture, and MBP expression pattern. I am new in the field of neuroscience. I am working on knockin mice of ALS...

10 October 2017 1,076 4 View

What is the reason for my problem in culturing C2C12 Cells in DHE Experiment?

Hi Everyone, I have a query regarding C2C12 Cells, my Colleague did a DHE (ROS detection experiment) in which its layers get disturbed, She seeded 100,000 Cells P# 39, in 6 well plate. And...

12 December 2014 9,330 4 View

Can anyone help with a HIT- T15 cell growth problem?

I am a student of MS, and have started work on HIT- T15 Cells. They were cryopreserved in 10% DMSO containing media. ATCC recommends Ham's F-12 media for its growth, however researchers are using...

06 June 2014 7,336 10 View

Can anyone help me with an INS-1 cells growth problem?

I have started to grow INS-1 Cells (P#49) in RPMI-1640 supplemented with pen-strep, Na- pyruvate, 50uM 2 Mercaptoethanol, 10mM HEPES PH7.4, 1mM Glutamine. I cryopreserved these cells a few months...

04 April 2014 7,663 18 View

Protein transfer on nitrocellulose membrane troubleshooting

I'm doing semi dry western blotting. I can see the bands right after the ponceau staining, but sometime it happens with my colleagues that bands were visible after the ponceau stain during...

08 August 2013 3,047 2 View

Need a protocol of insulin measurement from ins-1 cells

I have just enrolled in the diabetes research group. I will work on ins-1 cells of pancreatic beta cells. AGEs effect of these cells as well as by using novel AGEs inhibitors to observe the...

01 January 2013 4,936 2 View

Extracellular Protease of Bacillus licheniformis

I worked on Bacillus licheniformis extracellular protease, precipitation was carried out by 95% ethanol, precipitates was dissolved in 0.1M Tris -HCl buffer, sample was desalted by using G-25...

06 June 2012 4,937 7 View

Is it possible that a protease catalytic site is sensitive to both PMSF and EDTA?

I used inhibitors with different concentration EDTA and PMSF of 1 and 5mM, in the case of EDTA no activity was detected at 1 and 5mM, while in case of PMSF at 1mM activity was detected and no...

04 April 2012 3,425 7 View

Protease zymography

I have done zymography of amylase, in which i followed the SDS solution protocol, but now when i did it for protease, i again followed the same protocol, In the end i use 10 mM tris buffer for...

03 March 2012 2,604 4 View

role of T cell in the production of ig G

Hi i m a student of biotech, please guide me about it,,, polysaccharides (eg capsule) induce only humoral immunity,...

03 March 2011 9,424 3 View