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Questions related from Sedef Köseer
Currently I am trying to design TaqMan probe for Methylight experiment which will show the difference in methylation between 2 groups. These probes will contain 2-3 CpG sites in the promoter...
15 December 2017 3,219 1 View
I am currently trying to design a probe which should specifically bind to methylated CpGs in a certain gene promoter. The probe will probably cover 3-4 CpGs in its sequence, however, I do not know...
23 August 2017 5,197 0 View
Could a TaqMan probe hybridize specifically to its template at its 3'end even though 5' end of the probe has some unspecific nucleotides? And would it have any effects on the qPCR reaction efficiency?
21 August 2017 5,672 1 View
I am having difficulties with visualizing my PCR products (bisulfite-converted cell-free DNAs) in agarose gel. I know my primers are working (tested them with control DNAs), my positive controls...
09 April 2017 3,871 3 View
I am planning to perform Methylight to bisulfite-converted cell-free DNA isolated from blood(Serum/plasma). Can you please suggest which DNA Polymerase with 5' to 3' exonuclease activity works...
12 March 2017 8,385 2 View
I'm looking for circulating cell-free DNA isolation from either serum or plasma. After isolation I will perform Bisulfite conversion to these DNAs, so I need maximum amount. However there are lots...
02 March 2017 6,694 11 View
