Sebastian Alonso Quezada

2 Questions 4 Answers 0 Followers

Questions related from Sebastian Alonso Quezada

How to fixate with 95% Ethanol in fresh tissue cryostat sections?

Hi everybody When I get fresh frozen tissue (without fixating with PFA or formaldehyde, just snap frozen with OCT) sliced with the cryostat mounted in a slide it doesn't immediately get attached...

11 March 2016 8,108 1 View

Can someone advise me of an unbiased protocol for sampling the different layers of the brain cortex throughout a different fetal stages?

The thing is, I need to sample the different layers of a sheep cortex at different fetal stages, but I need to do it in an unbiased way, for I have to correlate gene expression from one point of...

25 January 2016 9,934 3 View