Sebastian Alonso Quezada

4 Questions 4 Answers 0 Followers

Questions related from Sebastian Alonso Quezada

Has anyone had success performing laser capture microdissection in non-PEN slides?

Hi everyone =) I'm gonna perform LCM again, and I was wondering if anyone has had success in performing it from normal (e.g. superfrost) slides. In the past I was recommended PEN slides and I was...

25 March 2019 8,623 1 View

OCT compound not freezing properly?

Recently I snap-froze some tissue sections to slice them using the cryostat, but when I took the blocks out of the -80 one of them didn't freeze properly, and was soft and fell apart when I tried...

21 March 2016 5,800 4 View

How to fixate with 95% Ethanol in fresh tissue cryostat sections?

Hi everybody When I get fresh frozen tissue (without fixating with PFA or formaldehyde, just snap frozen with OCT) sliced with the cryostat mounted in a slide it doesn't immediately get attached...

11 March 2016 8,129 1 View

Can someone advise me of an unbiased protocol for sampling the different layers of the brain cortex throughout a different fetal stages?

The thing is, I need to sample the different layers of a sheep cortex at different fetal stages, but I need to do it in an unbiased way, for I have to correlate gene expression from one point of...

25 January 2016 9,949 3 View