Saša Rezelj

4 Questions 2 Answers 0 Followers

Questions related from Saša Rezelj

Partially produced eGFP fusion protein?

With in vitro transcription translation I want to produce fusion protein with eGFP. I use the following linker: (..DELYK) - GSGSSGSGGS - (KDASA...). Is it possible that Gly-Ser linker is cleaved...

30 May 2016 276 3 View

How can I centrifugate giant unilamellar vesicles to prepare them by droplet transfer method?

I tried to centrifugate in an eppendorf tube, 30 min, 9000 xg, but the pellet sticks to plastic tube and the GUVs stick together (seen under microscope). They also stick to pipet tips. Should I...

12 November 2015 9,220 1 View

A hole in a test tube?

I would like to prepare GUVs (Giant Unilamerllar Vesicles) from inverted emulsion droplets. I found many protocols that describe the final step to collest GUVs by centrifugation: " GUVs were...

05 October 2015 5,784 1 View

How to dissolve mixture of amino acids at high concentrations?

We are trying to dissolve amino acids (for in vitro transcription and translation), each at 2M concentration and than mix them together to get 50mM mixture of amino acids. We encountered the...

01 January 1970 2,650 8 View