Sarah Sumner

4 Questions 2 Answers 0 Followers

Questions related from Sarah Sumner

Protocol for the detection of ROS in primary murine splenocytes (B cells specfically)?

Hi all, I've been attempting to measure ROS in stimulated and non-stimulated primary murine splenocytes (more specifically, B cells). I've tried both CellROX and H2DCFDA with little to no...

19 January 2021 5,745 6 View

Protocol for murine B cell Proliferation following stimulation?

I'm looking for a protocol to measure the proliferation of primary murine B cells following 72 hours of stimulation with anti-IgM, anti-CD40, LPS, and CD40 & IgM. I've tried CFSE (5 &...

26 November 2019 9,637 3 View

Are there any cell lines similar to DO11.01 cells that respond to MHCII presentation by C57BL/6 derived APCs?

Hi all, I’m looking for a T cell line that will respond to MHCII antigen presentation (OVA) by C57BL/6 derives cells. I know of DO11.10 cells but they’re derived from BALB/c mice which have a...

23 January 2019 2,877 1 View

What is a good protocol for the in vitro development of B cells (specifically pro-to pre-B cell Transition)?

I am looking for a protocol for in vitro B cell development. I want to utilize an in vitro culture system so that I can treat the cells with various compounds to see how they may effect...

01 January 1970 6,820 0 View