Saptha Vijayan

6 Questions 13 Answers 0 Followers

Questions related from Saptha Vijayan

Mice footpad histology protocol?

I have infected my mice with Mycobacterium marinum. The goal is to perform foot pad histology and do H&E and acid fast stain. Has any one performed footpad histology? Is there any standard...

07 July 2017 5,188 6 View

What if the insert exceeds the cloning capacity of the vector?

Can I put an insert size of 5.7kb into a vector which has a cloning capacity of 5.4kb. This vector is a retroviral vector system. I want to know if this is possible and what does the cloning...

04 April 2017 855 3 View

How to measure mice footpad swelling/inflammation?

I have infected the mice foot pad with Mycobacterium marinum and my goal is to monitor the footpad swelling using digital caliper and then check the footpad cfu after termination. My question is...

12 December 2016 7,404 14 View

What is the best protocol to check the expression of CD107a (LAMP-1) by Flow cytometry?

I need to titrate mouse CD107a antibody to be used for my future CD8+ T cell degranulation assays. After checking the literature, it is recommended to stimulate the cells with some antigen and add...

08 August 2016 9,345 7 View

With ChIP followed by qPCR, what sample should be used as standard for qPCR?

I am trying to optimize the ChIP process, which I would be later sending for sequencing. But before sending the samples for sequencing, I wanted to check for the presence of known DNA. So, I was...

08 August 2015 341 3 View

How can one transform Mycobacterium marinum?

I have been trying to get a mutant marinum for almost 4 months now and nothing seems to work so far (not even a single colony appears on the plate). I would really be happy is you guys could share...

11 November 2014 3,684 8 View