Sapir Ya'ar Bar

6 Questions 5 Answers 0 Followers

Questions related from Sapir Ya'ar Bar

Recommendation for reference management software?

Hello, Until now, we used Mendeley as reference management software when writing articles. recently we have tried to share the article online (google docs) so it will be easier for multiple people...

28 December 2022 8,317 1 View

Can I use more than 1 ug of RNA (3 ug) in verso cdna synthesis kit?

I want to use 3 ug of RNA, although the recommended amount is up to 1 ug. If it is possible, should change the amount of the other componenets respectively? I need the cDNA for qPCR. Thank you...

24 May 2021 475 6 View

How to create a metal binding curve for a metalloprotein using prism?

hello, I need to create a binding curve to measure the affinity of my enzyme to its zinc cofactor, to determine the Kd value. I'm using prism (graphpad) and there are a lot of options under the...

15 November 2020 2,184 3 View

Help with grid box and molecular docking using autosock 4.2?

hello, I'm trying to do molecular docking using pymol and autodock 4.2. I'm just learning how to use autodock now, using instructions online and youtube and it's quite difficult. I was trying to...

29 March 2020 9,150 2 View

standard curve for Size Exclusion Chromatography ?

Hello, I'm trying to deduce protein size based on its retention volume (45ml). unfortunately, we have a couple of columns for the SEC and we did a standard curve only for some of them, apparently...

16 October 2019 9,730 3 View

Possible explenation for excessive metal at the ICP-MS test ?

hello, we conducted an ICP-MS test to verify our enzyme is indeed a metalloenzyme containing 2 zinc ions. the expected ratio between zinc atoms to protein molecules was 2:1. the test showed a...

10 October 2019 5,814 4 View