Santanu Sasidharan

9 Questions 20 Answers 0 Followers

Questions related from Santanu Sasidharan

How do I find the Stein Volmer constant?

I have performed fluorescence studies to understand the binding of ligands to my protein. After measuring the fluorescence study and plotting F0/F vs [Q], I found that my plot is linearly...

30 October 2019 8,435 4 View

Kanamycin resistance cloning into eukaryotic vector?

I need to clone kanamycin resistance gene into a eukaryotic vector. Can I amplify the kanamycin resistant gene in pET28a(+) vector and clone it into the eukaryotic vector? Will it confer neomycin...

22 September 2019 1,293 5 View

Is BamH1(not hf) compatible with cutsmart buffer?

I have a double digestion to perform and i found that the second enzyme is compatible only in cutsmart buffer. Has anyone tried double digestion with BamH1 and Xma1 and cutamart buffer?

05 June 2019 7,491 3 View

Does EcoN1 site or Xho1 site gets modified after cloning?

I had restricted my insert and vector with EcoN1 and Xho1. After ligation, I had screened the colonies through colony PCr and found them positive. After plasmid extraction, I tried restricting...

31 May 2019 7,816 2 View

Why do I get multimeric PCR products?

When I run a PCR with a cloned plasmid, I get three bands ( product of 800 bp, dimeric around 1600 bp and trimeric around 2500 bp). This was for 25 cycles. When I run the same PCR for 35 cycles,...

01 December 2018 4,620 4 View

Modelling a modified residue in Modeller software

I have a modified amino acid in a template protein that I have chosen for modelling a query protein. How can I get the same modified residue in the modeled structure using Modeller software? I...

25 May 2017 1,037 3 View

Does anyone know about any procedure to extract sterile blood for blood agar medium in an easy way?

I am trying to prepare blood agar medium but i get contamination in the plates since the blood collected seems to be contaminated. Is there a way to get sterile blood or sterlize blood in an easy way?

24 January 2015 804 3 View

Problem with cloned colonies?

I had cloned a gene into pET28 and transformation was done on E.Coli DH5a. After transformation, the colonies were grown on Kanamycin plates of concentration 50ug/ml. The colonies were then picked...

01 January 1970 3,651 11 View

I am experiencing an error during my autodock dlg file analysis, what is this error about?

I had set an autodock run for docking an atp molecule to a kinase enzyme. I had set the number of GA runs to 1000. But, after the dock run had completed and I try to analyze the .dlg file, I am...

01 January 1970 2,386 10 View