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Questions related from Santanu Sasidharan
I have performed fluorescence studies to understand the binding of ligands to my protein. After measuring the fluorescence study and plotting F0/F vs [Q], I found that my plot is linearly...
30 October 2019 8,410 4 View
I need to clone kanamycin resistance gene into a eukaryotic vector. Can I amplify the kanamycin resistant gene in pET28a(+) vector and clone it into the eukaryotic vector? Will it confer neomycin...
22 September 2019 1,269 5 View
I have a double digestion to perform and i found that the second enzyme is compatible only in cutsmart buffer. Has anyone tried double digestion with BamH1 and Xma1 and cutamart buffer?
05 June 2019 7,471 3 View
When I run a PCR with a cloned plasmid, I get three bands ( product of 800 bp, dimeric around 1600 bp and trimeric around 2500 bp). This was for 25 cycles. When I run the same PCR for 35 cycles,...
01 December 2018 4,598 4 View
I have a modified amino acid in a template protein that I have chosen for modelling a query protein. How can I get the same modified residue in the modeled structure using Modeller software? I...
25 May 2017 1,019 3 View
I had cloned a gene into pET28 and transformation was done on E.Coli DH5a. After transformation, the colonies were grown on Kanamycin plates of concentration 50ug/ml. The colonies were then picked...
01 January 1970 3,639 11 View
I had set an autodock run for docking an atp molecule to a kinase enzyme. I had set the number of GA runs to 1000. But, after the dock run had completed and I try to analyze the .dlg file, I am...
01 January 1970 2,377 10 View
