Sandeep K. Panda

7 Questions 10 Answers 0 Followers

Questions related from Sandeep K. Panda

How many different origin of replication (ori) sequences can I have in a plasmid?

Multiple ori sequences pertaining to different hosts are usually present in a plasmid. But how many different ones can I have, without causing problems? If problems do get created, what kind of...

11 November 2019 678 3 View

How can you predict the translation initiation rate from a sequence upsteam of start codon in a mRNA transcript?

What are the major factors that influence the above? Are there any good bioinformatic prediction tools for the same?

07 July 2019 891 3 View

Is it ok to have the same bacterial terminator sequence in 2 or 3 different places (downsteam of different genes) in the plasmid?

1. Do I need different terminators or is it ok to have the same terminator downsteam of different genes? 2. If its not ok, what kind of problems does the plasmid have?

12 December 2018 2,446 4 View

What are the different ways to identify a promoter for a specific stage of bacterial life cycle?

Let us say a bacteria has a generation time of 20 minutes. How do we find the different promoters which are active at the 15th minute (for example)?

08 August 2017 1,196 2 View

What are the top ten journals ranked based on JIF (Thompson Reuters) 2016? What are the top ten papers based on citation index 2016?

Kindly provide the source too.

03 March 2017 1,711 2 View

Yeast vs Plant cell - which is better for the production of recombinant proteins?

Kindly elaborate on pros and cons of both, if possible. I'm interested in understanding the differences in levels of recombinant protein produced, ease of production and ease of genetic...

03 March 2017 828 10 View

How can I design antibodies in-silico from only the antigen peptide sequences?

The protein I have selected from my screening didn't have a known 3D structure, so I had to generate it. Some of the servers I used are - Phyre, I-TASSER, Quark, CABS-fold, RaptorX, Robetta.. but...

04 April 2016 8,911 6 View