14 Questions 24 Answers 0 Followers
Questions related from Sandeep Rai Kaushik
What are the best markers for Erythropoiesis in mice? There are several markers like CD45, CD34, CD117, CD105, CD235a, CD71 based on specific stages of erythropoietic cells. Which of the above or...
20 October 2020 3,632 8 View
Hi, what is the best Duodenal Enterocyte marker for 1. Immunohistochemistry 2. Western blotting 3. Flow-cytometry/FACS
20 October 2020 5,723 3 View
Hi, I would like to develop an animal (mice) model for the iron overload conditions. As Diet based methods take a longer time to develop, I would like to use the injectibles options. Please...
12 October 2020 4,562 1 View
Hi, I am using serum samples for western blotting. I have used different antibodies (for different target proteins) and most of them are monoclonal. I am getting unusual results like a band around...
16 December 2019 1,965 6 View
Hi All, I'm planning for an RNAi experiment. I have few questions/doubts. Which RNAi technology is better ( I'm planning for siRNA) and why? Which siRNA is better (planning Silencer® Select siRNA...
06 June 2019 4,329 11 View
I have serum samples and want to use ICP-MS to measure few elements like Cu, Zn, Fe , Se. I have procured Multi-element standard from Sigma and also Single element standard for one element. I have...
05 February 2019 2,373 3 View
I want to use ICP MS for metal ion quantification from Serum samples. Please suggest which water can be used for dilution ?(Least expensive but for best results). Where I can buy ICP-MS grade...
20 April 2018 5,901 2 View
Hi, I am using A549 cells. I have taken a cryovial from -80 to revive the cells. I have revived at 37 for around 2 min and then gave two media wash. then seeded all the cells in a T-25 flask...
03 October 2016 9,847 3 View
I have run an SDS PAGE and after Silver-staining, I am getting extra thin lanes (Vertical) bifurcated from main lane. What could be the possible expalnations for these thin lanes??
22 August 2016 1,929 7 View
I have extracted the Genomic DNA from Candida albicans. Instead of a High size band i got a single very bright very below in gel. What it can be and why it happened?Note: I did not use RNAse...
30 May 2016 2,851 10 View
I'm doing iron quantification using Ferrozine assay but very low or negligible absorbance in serum samples. I am getting very good absorbance in standards. I am using Ascorbic acid as reducing...
03 March 2016 5,649 2 View
How to differentiate THP-1 Monocyte to Macrophages? What conc. of PMA has to be used? How long it takes to differentiate? Please share a detailed protocol.
01 January 1970 2,095 2 View
I have a few questions about membrane proteins especially phagosomal membrane proteins. Can these proteins be secreted in circulation? If Yes, how can we show that? Can these proteins be secreted...
01 January 1970 7,996 2 View
Generally, IFN-g levels are very high in tuberculosis patients. And it is expected that pro-inflammatory cytokines will be at higher end while anti-inflammatory will be at the lower end. I have...
01 January 1970 9,265 3 View
