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Questions related from Saloni Rishi
For fungal identification I will be using TEF ALPHA tricho primer which has Tm 52°C. I wanted to know the PCR conditions as even by setting the annealing temperature at 48°C I was getting multiple...
22 February 2022 9,753 3 View
I wanted to perform PCR for fungal identification using TEF ALPHA tricho-F and R primer with Tm 52 as given by the seller. But even after performing PCR at 48° and 56°C (annealing temp) I got...
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What does band area indicate in FT-IR (absorbance). I have different band area at same wavelength in control vs treated microbial culture. Is it correct to calculate increase or decrease in band...
02 February 2022 8,863 3 View
can anyone suggest me the best pcr conditions for 16s 27F and 1392R primers as I am getting multiple bands. I have thermo as well as genetix pcr master mix.
08 October 2021 3,545 2 View
I wanted to perform rhodanese enzyme assay using salt potassium ferricyanide (K3Fe(CN)6). The assay is based on the products formed in the reaction but I can't get the reaction i.e what product...
27 January 2021 8,232 4 View
