Salma Abdelmaboud

2 Questions 3 Answers 0 Followers

Questions related from Salma Abdelmaboud

How to dry slides after cover slipping?

Hello, i have immunoflurecent staining for brain tissues on slides. I used hydrophobic pen and hard set mountain media. The slides kept overnight to dry for two days but they’re still wet?

12 October 2023 8,341 2 View

Hi, I have collected brains for IHC in PBS instead of PFA, and after 24h I changed media to 30%sucrose. Can I fix that with putting them in PFA again?

I have collected brains for IHC in PBS instead of PFA, and after 24h I changed media to 30%sucrose. Can I fix that with putting them in PFA?

13 February 2023 5,514 4 View