Saadiya Zia

4 Questions 4 Answers 0 Followers

Questions related from Saadiya Zia

I need guidance how to design primers for miRNA?

The size miRNA is 89 bps and the primers I have designed (using traditional method for designing gene primers) should give an amplified product of 71 bps. But I am getting product of higher...

24 May 2024 7,439 3 View

Melt curve of CAT25 Microsatellite marker shows two peaks while single band appears on the gel. What could be the most likely reason?

I am working on CAT25 microsatellite biomarker. The melt curve shows two peaks while single band appears on the gel. Primers are taken from the published research papers. The DNA is of healthy...

16 May 2023 3,756 0 View

Isolation of single cell suspension without enrichment?

We are isolating single cell suspension from breast tumor tissues by enzymatic digestion (trypsin digestion). But I am getting low cell count after passing the suspension through 40 micron filter,...

27 March 2019 5,575 3 View

I tried to dissolve my drug in 0.1% dmso, but it wont dissolved. I dont have much drug, How can i solubilize the drug?

i added 500ul of 0.1% dmso in 5 mg of the drug

03 April 2017 9,220 4 View