S M Abdus Salam

3 Questions 1 Answers 0 Followers

Questions related from S M Abdus Salam

How to get correct band (S100A8) in western blot?

I have used 7 cell line (from left to right). THP1 is the positive control. I want to detect S100A8 protein (molecular weight ~11kda). My positive control cell line always give me exact band, but...

22 November 2024 4,910 0 View

How to separate cells after direct co-culture?

I want to do a direct co-culture of cancer cells and fibroblasts. After doing co-culture, I want to get back my cancer cells and fibroblast for further downstream analysis. So, how to separate...

18 March 2022 560 3 View

How to separate homogenous cancer associated fibroblast cells from heterogenous cancer tissue of mouse/human?

I am not interested of FACS, MACS, or any other process that effect on cell morphology. Actually I need a procedure that retain the cells original characteristics with highly pure cells. If any...

18 October 2021 5,864 0 View