Ryan Chaparian

4 Questions 12 Answers 0 Followers

Questions related from Ryan Chaparian

'V' shaped bands in EMSA?

I'm having issues regarding the morphology of the bands produced in my electrophoretic gel mobility shift assays (EMSAs). At high enough concentrations of protein, the bands begin to take the...

10 October 2018 3,118 9 View

A very odd Western blot transfer issue...?

Hi all, I am having a very odd issue regarding my Western blot transfers. When I perform a transfer, sometimes my bands diffuse across huge areas of the membrane and become 'wave-like.' I've...

01 January 2018 8,608 10 View

My protein is heat/SDS/urea resistant: are there any alternative ways to denature proteins?

Whenever I run my protein (MWmonomer = ~12 kDa) on an SDS-PAGE gel, I always see another band present at ~25 kDa (roughly the size of the dimer). I suspect that the band is the dimeric form of the...

03 March 2016 5,948 14 View

in SDS-PAGE, how can I prevent well walls from collapsing?

I use the CBS Scientific gel assembly apparatus (http://www.cbsscientific.com/verticalmini-gelelectrophoresissystemaccessories.aspx) to make polyacrylamide gels. Lately, I have been experiencing a...

01 January 2015 7,212 7 View