Rucsanda Pinteac

6 Questions 6 Answers 0 Followers

Questions related from Rucsanda Pinteac

How can I compare my neurons basal microarrays transcriptome with a curated neuronal/brain expression database?

Hi dear colleagues, Thank you for reading my question. I'm struggling to compare my basal transcriptome microarray data (Clariom S, human, Affymetrix) versus some brain or neuronal database to...

13 July 2023 3,872 0 View

Transfection of iPS-derived neurons?

Hi everyone, I'm trying to visualize dendritic spines in iPS-derived neurons by transfecting a GFP-actin plasmid. My goal is to achieve a low-efficiency transfection to analyze single neurons...

08 March 2022 9,165 0 View

Is anyone working with StemCell Forebrain Differentiation and Maturation kits?

Hi everyone! I'm working with iPSC-derived neurons right now and differentiating my neurons using the StemCell Forebrain Differentiation and Maturation kits. I induce my NPCs using StemCell Smadi...

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Permeabilization Step TUNEL in adherent cells?

Hi everyone! I'm trying to quantify apoptosis using TUNEL (Roche, 11684795910) and it works great exceptuating that in the permeabilization step (Sodium citrate 0.1%, pH 6, 2' on ice) my cells...

25 September 2020 8,129 4 View

How can I detect cleaved-caspase 3 by western blotting?

Hi everyone, I'm not able to detect cleaved caspase-3 by western blotting using the ab13847 antibody. My wb input are lysed primary cortical neurons treated with staurosporine 1 uM for 4 and 6...

23 September 2019 3,482 3 View

Which is the best protocol to transfect astrocytes (primary cell culture) with Lipofectamine 3000?

Hi everyone, We are trying to transfect astrocytes (24 wells plate) and we followed the original protocol with the high concentration for lipofectamine 3000 (1,5 uL). We had a high amount of...

09 March 2018 1,625 9 View