Rouaa Shaheen

4 Questions 4 Answers 0 Followers

Questions related from Rouaa Shaheen

How can I treat cells with 0.1 DMSO solution?

I bought an active compound as a powder and dissolved it in 100 percent DMSO as stated in the data sheet. This is my stock solution. Now I'm planning to do experiments on cells but I read that the...

24 February 2023 4,956 0 View

How to perform HDDP assay in 96-well plate?

I'm planning to perform dpph antioxidant assay on my active compounds and I already have the protocol for that. I'm aware that i should include a blank and a positive control but I have zero idea...

23 February 2023 6,418 6 View

Any advice for my docking result?

this my first attempt to dock a protein and a ligand (plant saponin) using autodock vina. the screenshot I attached is the molecule after docking. does it look normal? my ligand looks so complex...

26 December 2022 5,080 5 View

What is the optimal incubation time for primary and secondary antibodies in western blot?

I joined a lab recently and the work here is mostly western blotting. My lab mates incubate primary antibodies for 2-3 days at 4 degrees Celsius and secondary antibodies for 1 day (overnight) at 4...

23 November 2022 8,582 8 View