Roshan Kumar

5 Questions 5 Answers 0 Followers

Questions related from Roshan Kumar

Multiplexing of gRNAs using seamless cloning strategy?

Hello everyone I'm just curious to know if anyone have used HiFi DNA assembly kit (NEB) for carrying out multiplexing of gRNAs. Actually, I am trying to do multiplexing of gRNAs scaffold regions,...

21 June 2018 2,249 4 View

E. coli culture gets granulated after addition of IPTG

I am using Rosetta gami (DE3) cells for purification of recombinant protein. Addition of IPTG (1 mM) and overnight incubation of culture at 20C leads to formation of granules which is quite...

19 May 2017 6,098 5 View

Phenotypic or biochemical evidences to confers sensitivity or resistance under stress conditions?

Is it necessary to get phenotypic or morphological changes, when comparing the wild type and transgenic lines under various abiotic stress condition or only biochemical evidences such as proline...

06 June 2016 9,296 2 View

How do measure growth parameters in Brassica crops?

Which phase or time period are generally recommended to measure the growth parameters of reproductive stages (flower and pod) in Brassica crops?

13 January 2016 3,980 5 View

Why does hoagland nutrient solution get precipitated after addition of CuSo4?

I am using hoagland solution of pH 5.6-5.8. but after addition of CuSo4.5H2O turbidity was observed in the nutrient solution.

12 June 2015 5,366 3 View