Rony Fernando Orobio Hurtado

4 Questions 6 Answers 0 Followers

Questions related from Rony Fernando Orobio Hurtado

Any suggestions about SOB medium: Autoclaving MgCl2 or MgSO4? Also, is MgSO4 needed?

Hello. I have two doubts: 1) I am checking protocols for preparing SOB medium. Some of them (including Sambrook et al 2003) suggest autoclaving the broth prior to adding MgCl2 or MgSO4. Some other...

12 December 2015 9,147 2 View

Which one is better, the Zeocin or kanamycin version of the pZErO plasmids?

Hello. I am defining a protocol to create a library in E coli. There exist two versions of the zero background plasmid (pZErO). One with kan resistance and one with zeocin resistance. Does anyone...

02 February 2015 10,069 4 View

Is there any software to set baseline and find Cqs from raw CFX96 fluorescence data?

We are experiencing some troubles with the analysis of some data from several genes in CFX Manager. We want to adjust the baseline from some target genes data, as it was automatically set as to...

04 April 2014 1,049 2 View

Can someone share a copy of an old GeNorm software for qPCR ref. gen ranking?

Hello, everyone. I am planning the analysis for a experiment of gene expression through qRTPCR. I would like to try the GeNorm softare amongst others to assess the stability of my reference gene...

07 July 2013 2,520 2 View