Ronny Fischer

4 Questions 11 Answers 0 Followers

Questions related from Ronny Fischer

How do i combine the results from consecutive mtt proliferation assays?

This might be an easy one for those who do it on a regular basis...I have performed an XTT proliferation assay on two different cell lines with 6 different concentrations of a substance. I...

08 August 2017 8,340 5 View

How can i illustrate the difference in histological staining intensities?

I am staining serial sections with different pretreatments. Section 1: pretreatment A followed by staining X Section 2: No pretreatment followed by staining X Section 3: pretreatment B followed by...

08 August 2017 2,861 4 View

Can i change pH of buffer after cleaving Hyaluronan with Hyaluronidase?

For an experiment i need the small cleavage products of Hyaluronan, meaning that i need especially the activity of Hyal-1 in the product H3884 of Sigma. I guess this is why standard protocols use...

04 April 2017 5,876 2 View

After addition of protease inhibitor protein concentration (Bradford) decreased when sample was centrifuged but increased when filtered. Why is that?

Sample: colon lavage I took 2 times 1ml of the same sample. After that one sample was centrifuged (14000 rpm, 4°C) and the other was filtered (0,4µ pore size). I took 2µl of each sample and...

07 July 2016 2,726 12 View