Romain Pacaud

5 Questions 9 Answers 0 Followers

Questions related from Romain Pacaud

What do I still have multiple bands after isolating each bands through Gel extraction ?

Hi, I'm trying to develop a KO cell line from an established cancer cell line. My gene of interest is present in 3 copies in this cell line. I'm using a multi-sgRNAs technique to increase my...

02 December 2021 8,731 5 View

Can you explain to me the principle of Loss of heterozygosity (LOH) ?

Hi everybody, I am little bit confused about the loss of heterozygosity term. If I understand, we can say there is a LOH when one allele is lost (a wild type allele) in the case of the other...

25 January 2017 9,061 12 View

Why is BRCA mutation a higher factor risk for breast and ovarian cancer than other type of cancers ?

BRCA 1 and 2 are involved in the HR DNA repair pathway and they have an ubiquitous distribution in the whole body. I just wonder myself why is there a higher risk of breast or/and ovarian cancer...

17 January 2017 4,306 5 View

Why is the DNA concentration higher in my Negative control sample than in my IP antibody test sample in a ChIP experiment ?

Hi everyone, I have some trouble in my ChIP experiments. I want to study the recruitment of transcription factor and DNMTs on DNA. For this, I cross-link cell with 1% final of formaldehyde during...

12 November 2014 2,132 4 View

Stringency of the single guide RNA for classic CRISPR/Cas9 system

Hi everybody, I have a relatively simple question but couldn't find an exact answer. If you want to KO a gene with a CRISPR/Cas9 system, and the gene of interest as a paralogous counterpart gene,...

01 January 1970 4,699 0 View