Romain Pacaud

10 Questions 14 Answers 0 Followers

Questions related from Romain Pacaud

Why our copy number analysis fluctuates with Quantibrite PE beads?

Hi, I have a question about FACS and copy number estimation of a protein of interest. We have a protein of interest (on the membrane surface) that we are studying and we have an antibody coupled...

22 February 2023 1,966 0 View

What do I still have multiple bands after isolating each bands through Gel extraction ?

Hi, I'm trying to develop a KO cell line from an established cancer cell line. My gene of interest is present in 3 copies in this cell line. I'm using a multi-sgRNAs technique to increase my...

02 December 2021 8,807 5 View

How to analyze PLA slides on Zeiss spinning disk confocal only in the nuclei?

Hi! I did an experiment involving the Proximity Ligation in situ assay (PLA) and I analyzed my samples with a confocal microscope. I have two channel: one DAPI for the nuclei and one Texas Red for...

09 September 2020 7,538 1 View

Is it possible histones undergo deacetylation or dephosphorylation without the action of dedicated eraser proteins ?

Hi everybody, my question is about the histone mark dynamic. The Methyl group on the carbon 5 of a cytosine can be removed spontaneously without the intervention of demethylating actors (like TETs...

28 June 2017 3,742 3 View

What is the link between BRCA2 and ER receptor ?

Hi everybody, BRCA-mutation related breast cancers are known to be (in a majority of case), linked to the ER status. Indeed, literature reports BRCA1 cancers are mostly ER- and BRCA2 are mostly...

22 June 2017 5,869 6 View

Why inhibition of 53BP1 improves snesitivity in cells after IR and promotes PARPi resistance ?

Hi everybody, 53BP1 is known to block single strand resection which is an essential mechanism for the Homologous Repair DNA pathway, and by consequence, promotes the NHEJ pathway. In BRCA1 mutated...

24 May 2017 2,194 1 View

Can you explain to me the principle of Loss of heterozygosity (LOH) ?

Hi everybody, I am little bit confused about the loss of heterozygosity term. If I understand, we can say there is a LOH when one allele is lost (a wild type allele) in the case of the other...

25 January 2017 9,179 12 View

Why is BRCA mutation a higher factor risk for breast and ovarian cancer than other type of cancers ?

BRCA 1 and 2 are involved in the HR DNA repair pathway and they have an ubiquitous distribution in the whole body. I just wonder myself why is there a higher risk of breast or/and ovarian cancer...

17 January 2017 4,406 5 View

Why is the DNA concentration higher in my Negative control sample than in my IP antibody test sample in a ChIP experiment ?

Hi everyone, I have some trouble in my ChIP experiments. I want to study the recruitment of transcription factor and DNMTs on DNA. For this, I cross-link cell with 1% final of formaldehyde during...

12 November 2014 2,224 4 View

Stringency of the single guide RNA for classic CRISPR/Cas9 system

Hi everybody, I have a relatively simple question but couldn't find an exact answer. If you want to KO a gene with a CRISPR/Cas9 system, and the gene of interest as a paralogous counterpart gene,...

01 January 1970 4,754 0 View