Robin Moll

4 Questions 12 Answers 0 Followers

Questions related from Robin Moll

Any Nuclei extraction from Nematode that are not super labour intensive?

I am culturing 20 species of Caenorhabditis and require a technique for nuclei extraction for HiC sequencing. There are many methods out there that take from 15 minutes to a day's worth of...

18 January 2020 3,890 1 View

Is there a reduction in the sensitivity of PCR when using universal primers with degenerate sequences?

I am using a pair of primers each oligo has a degeneracy of 4, so an overall degeneracy of 8 when they are used together. The primers are for the amplification of 16S rDNA so are being used to...

20 May 2015 8,393 3 View

What values do we use for statistical testing of qPCR delta delta ct (ddCt)?

I have performed ddct with mean ct values for 15 test replicates and mean ct values for 11 calibrator samples. I have all my values for ddct so it's not calculating the my expression fold change...

30 April 2014 1,203 9 View

Should experimental triplicates have very similar Ct values?

I have performed an experiment in which THP-1 cells were exposed to a bacterial strain (heat killed) as a model of infection. I decided upon an MOI of 100 and performed the experiment with three...

29 January 2014 9,579 12 View