Robin Moll

6 Questions 15 Answers 0 Followers

Questions related from Robin Moll

Any Nuclei extraction from Nematode that are not super labour intensive?

I am culturing 20 species of Caenorhabditis and require a technique for nuclei extraction for HiC sequencing. There are many methods out there that take from 15 minutes to a day's worth of...

18 January 2020 3,942 1 View

Is there a reduction in the sensitivity of PCR when using universal primers with degenerate sequences?

I am using a pair of primers each oligo has a degeneracy of 4, so an overall degeneracy of 8 when they are used together. The primers are for the amplification of 16S rDNA so are being used to...

20 May 2015 8,436 3 View

Is a regression line plot for (cfu)/ml vs OD600 representative of that bacteria in all media?

I have constructed an OD600 vs cfu/ml regression line plot to find the line of best fit allowing a conversion from optical density to cell number. I'd like to know if this conversion for one...

06 May 2015 3,967 4 View

What values do we use for statistical testing of qPCR delta delta ct (ddCt)?

I have performed ddct with mean ct values for 15 test replicates and mean ct values for 11 calibrator samples. I have all my values for ddct so it's not calculating the my expression fold change...

30 April 2014 1,263 9 View

Should experimental triplicates have very similar Ct values?

I have performed an experiment in which THP-1 cells were exposed to a bacterial strain (heat killed) as a model of infection. I decided upon an MOI of 100 and performed the experiment with three...

29 January 2014 9,626 12 View

What's the ideal PMA conc for differentiating THP-1 cells?

I have seen within a number of different papers a big range of concentrations PMA utilised for THP1 differentiation. Some use quite weak conc and wait a few days for differentiation while others...

23 July 2013 7,947 2 View