Ria Fajarwati Kastian

4 Questions 11 Answers 0 Followers

Questions related from Ria Fajarwati Kastian

How to get a consisten staining of DiI staining for brain slice to visualise dendritic spines in hippocampus?

Dear all, I am looking for a great experience from who has an expert skill for DiI staining. I have done this technique for almost 6 months, however my data always not consistent. "Not consistent"...

06 June 2016 6,974 4 View

Does anyone have experience with in vivo electroporation in brain mice with hippocampus targeted?

Im trying to do in vivo electroporation (EGFP injection) in P0 brain mice which targeted into hippocampus. After 5 days, GFP positive cells are look localise in hippocampus. But, I wondering about...

09 September 2015 3,181 1 View

Dear all, is lipofectamine toxic to primary neuronal cells?

I transfected mature neuron cells by lipofectamine 2000, DIV 13 and staining at DIV 16. But, unfortunately, there was some positive transfected cells show abnormality, such as green fluorosence...

08 August 2015 239 12 View

Can anyone help with flow cytometry cell cycle on t47d treated with doxorubicin?

I treated t47d cell line with 1/2 IC50 of doxorubicin, then I analyzed it in flow cytometry to see the effect of cell cycle of t47d. But from the result, I get a different histogram from others....

04 April 2014 6,887 2 View