Reza Rezaei

5 Questions 11 Answers 0 Followers

Questions related from Reza Rezaei

Is it possible to culture several cell lines in the same plate/dish/flask with complete cell separation?

I'm trying to screen the effect of a toxic agent. I need to see its effect on a few different cell lines with different degrees of resistance to this compound. I would like to possibly culture...

01 January 2021 5,981 3 View

What is your recommended miRNA-seq Data Analysis workflow?

I have some miRNA-seq raw data and I need to search for miRNA:mRNA interaction networks. After doing a quick literature review, I found several tools including mirDeep2, mirArma-seq, and other...

03 July 2020 8,757 0 View

Do i need to add ribosome binding site or other sequences to my antibiotic resistance gene ORF for an allelic exchange in a streptococcus strain?

I want to knock out a gene in a Streptococcus bacterial strain and I'd like to use allelic exchange method. I studied in an article that it is better to use an antibiotic resistance gene without a...

13 September 2017 1,230 0 View

What is the effect of a without-FBS medium on cells?

i am doing a Osteogenic differentiation for 21 days, but the growth of my mesenchymal stem cells isn't stoped . i am worry about over growth of them and after that starting the apoptosis procedure...

15 November 2016 3,306 8 View

The best way for separating cells from gel-like scaffold ?

I have a kind of agarose-based scaffold and the size of that is like a well of 6 wells plate and I want to separate cells from scaffold for adding lysing solution and using a kit for extracting...

29 July 2016 194 8 View