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I am currently trying to purify proteins from different insect cell lines and baculoviruses. One of the criteria I want to check is the amount of host cell protein (HCP) in the purified sample....
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I have been trying to use a CsCl gradient to purify an intracellular virus. However after centrifuging at 100000xg for over 20 hours there are no bands and the cellular proteins are distributed...
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