Ray Radha Sargunam

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Questions related from Ray Radha Sargunam

Can anyone help me in preparing Ketonic diet? Since it is approx 70 % Fat, i am facing difficulty in administering the prepared Ketogenic diet?

I have prepared a ketogenic diet consisting of 70% fat.. I am facing problem in administering it in animals ad libitum because of its paste like consistency. Can anyone really help me solve this...

07 October 2016 7,809 5 View

Does ligation of internal carotid artery or mechanical blockade of internal carotid artery clinically simulate stroke ?

I would like to know whether blockade of internal carotid artery using a Bulldog clamp or ligation of internal carotid artery a valid mode for Stroke? Does this method make it superior to...

11 August 2016 485 6 View

Hi everyone, I am having a problem with hippocampal sections in Paraffin sectioning? Can anyone help?

I dissect the animal, intracardially perfuse with 0.9% Saline, then with few ml of 4% PFA intracardially. Then i remove the brain and fix it in 10 % formalin for 2-3 days after which i embed the...

16 February 2016 7,398 8 View

Can anyone suggest to me how to learn Middle cerebral artery occlusion (MCAo) model for stroke?

I can perform, isolate the external and internal carotid artery. After this, what precautions should i take into consideration in the induction of the model? Can anyone help me with videos of MCAo...

02 February 2016 2,756 4 View

What are the problems associated with paraformaldehyde perfused cryosections of mouse brain?

I perfused mice intracardially with 2%paraformaldehyde +2%glutaraldehyde, and stored in similar medium for 3-4months. we washed brain twice or thrise with PBS solution before cryosectionig...

21 September 2015 8,576 2 View