Please suggest HPLC method for estimation of saponin.
Extraction method?
estimation method?
HPLC column?
For saponin extraction----Use Methanol for extraction partioning with saturated butanol followed by column chrm dianionHP- 20 followed by C-18
For detection on HPLC use ACN:Water column C-18 at 210nm at PDA or you can use ELSD detector.
You can see our paper on saponins.
Gudluck
:)
Dear Robin,
thanks a lot for you valuable suggestion. i got that paper
Hi Robin,
You can also check this paper for the extraction and quantification of saponin using HPLC.
Gnoatto SC, Schenkel EP, Bassani VL. HPLC Method to assay total saponins in Ilex paraguariensis aqueous extract. J Braz Chem Soc. 2005;16:723–6.
copy the link below to check the paper.
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532005000500007
I hope this helps.
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
I have not addede any paper yet but it has selected 3 papers which are not mine in my account. i want to delete that information. please help me
30 July 2024 3,743 0 View
The threshold voltage (Vth) of a MOS device plays a crucial role for its operation. At the same time, noise is an intrinsic factor. So how noise (flicker or thermal) change with the change in...
29 July 2024 3,246 0 View
If you don't understand my question let me know
21 July 2024 563 1 View
I am working on scale development in behavioral finance by undertaking a mixed-method approach using the exploratory sequential design. The phenomenon has diverse meanings in existing literature...
20 July 2024 9,153 11 View
I need to convert Available phosphorus into total phosphorus
18 July 2024 6,799 3 View
Bioinformatics tools like peptide cutter
15 July 2024 6,453 1 View
The burden of obesity is enormous. The greater challenge lies in accurately diagnosing obesity at the right time, reflecting the true increase in visceral fat. BMI may not accurately represent...
14 July 2024 8,771 4 View
How does high humidity affect the growth and development of crops?
10 July 2024 7,258 20 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View
I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...
31 July 2024 8,309 0 View
I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...
30 July 2024 4,267 2 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
I am doing research on wear mechanism of the additive manufactured metal parts under dry condition. Please share the reference to start the analytical modelling of the wear prediction.
29 July 2024 6,115 0 View
Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...
28 July 2024 540 2 View