Ramona Salcedo Price

3 Questions 14 Answers 0 Followers

Questions related from Ramona Salcedo Price

What type of biosafety cabinet, glove box or fume hood, and filters does your lab use for aflatoxin (in solution) studies?

I will be using aflatoxin b1 solution for in vitro studies. However, my institution suggests that I may need a carbon filter. Currently, I have access to a class II type A2 biosafety cabinet and a...

08 August 2017 947 1 View

The case of the disappearing proteins from an immunoblot.

I can successfully probe for 1 protein but NOTHING else after (actin, gapdh or tubulin). I combined the primary antibodies of actin and my protein of interest and this seems to skirt around the...

04 April 2013 7,109 7 View

What are the best options to identify Th17 cells in paraffin-embedded tissue? Any thoughts?

We are using the pten mouse model of prostate cancer and we'd like to determine if T-cells infiltrate the prostate and the microenvironment in the context of our experimental conditions. IHC was...

04 April 2013 1,318 9 View