7 Questions 9 Answers 0 Followers
Questions related from Ramón Soto
Hi all, I am working on a tricky protein. I surpassed some initial aggregation behaviour by adding 0.3% CHAPS and 1mM TCEP, buffer in which is happy until the removal of the SUMO tag, then it...
12 December 2018 2,860 3 View
Hi all, a question for the protein purification masters here. After protein elution from a Ni column, some people simply dilute the sample down to decrease the imidazole concentration. I have...
10 October 2018 2,533 10 View
Dear all, When titrating a high affinity ligand I am getting Kd values that are very close to the total protein concentration in the system. My understanding on this matter is that, under such...
10 October 2018 3,072 16 View
Hi all, I would like to know whether the coexistence of two plasmids can lead to a low plasmid copy number of each of them? Assuming that these plasmids have a compatible origin of replication. We...
04 April 2015 4,954 4 View
I transformed by BCG culture with my gene of interest, and after growth I set up an experiment with a drug that is used as a control for the expression of my gene: if my gene is overexpressed,...
11 November 2014 4,572 1 View
I am slightly concerned about my overexpression studies where one of my BCG strains should confer resistance to my control compound, but it seems like either the compound cannot enter the cell or...
11 November 2014 308 2 View
I am reviewing relevant literature for DNA extraction from this bacterium. Considering that my objective is cloning, which is the best protocol to be followed? Which kind of culturing techniques,...
02 February 2014 1,852 4 View