Ramin Lotfi

8 Questions 16 Answers 0 Followers

Questions related from Ramin Lotfi

What is the reason of sensitivity difference of ELISA kits relevant to various companies?

Hi every body, I measured serum level of resolvin D1 (RvD1) by an ELISA kit from EASTBIOPHARM company, which its sensitivity and assay range was ng/ml. On the other hand, ample studies and papers...

16 August 2018 1,877 5 View

What is the difference between amount of lipid-derived meditors (LMs) such as resolvins (Rvs) in serum and plasma?

Lipid-derived mediators (LMs) such as resolvins (Rvs) are produced from essential fatty acids (EFAs) and also are present in serum and plasma. Now I am curious to know that whether the amount of...

27 January 2018 6,265 0 View

Is there a way to check the contamination of primers with cDNA?

I want to check the contamination of primers with cDNA, because in real time PCR their peaks are not very good and have extra peaks. Moreover, the negative control without cDNA (NTC) has a peak...

12 January 2018 4,511 10 View

Why do I get two or three peaks in melting curve of real time PCR?

Hi every body, I want to set up real time PCR for one gene, but after doing it I get two or three peaks in melting curve of real time PCR. The designed primers for my gene is very specific. After...

04 January 2018 1,538 13 View

How much resolvin (e.g. RvE1 & RvD1) is in the human serum?

Hi everyone, The resolvins (Rvs) are produced from omega-3 fatty acids (such as EPA & DHA) and are involved in the process of resolving inflammation. Now I am curious that how much resolvin...

21 December 2017 3,826 0 View

Which cells in the immune system express the GPR120 and PPAR-γ genes?

Hi everyone, I want to investigate the expression of two genes (GPR120 and PPAR-γ) in PBMCs. Now I want to know how much of the two genes are in leukocytes and which cells express this two...

12 December 2017 9,214 2 View

What is the problem in set up of my PCR?

Hi everyone, I want to set up PCR for two genes (GPR120 and PPAR-γ). I twice designed two specific primer for each of this two genes by online primer design sites, but unfortunatly none of two...

06 December 2017 4,768 6 View

Does the ELISA appropriate method for measure of Lipid mediators derived from Omega-3 Fatty acids such as Resolvins?

I want to use ELISA to measure of Resolvin E1, but I know that the best method for measure it is LCMSMS, But I don't have access to LCMSMS. Now I Would like to know whether the ELISA for the...

03 January 2017 5,445 2 View