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I am working through the challenges of cell isolation from the rat heart. I am using VSMC media and endothelial cell media. In a homogeneous mixture of cells, would these media favor only the...
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Hello, we have been sacrificing mice and rats using cardiac puncture. I am curious if anyone knows of publications or has had personal experience relating to potentially altered protein...
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Once cells have been collected in RIPA, before centrifugation (16,000g for 20 min) should cells be sonicated? Does sonication improve protein yield?
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Does heating influence RNA quality? My understanding was the RNA should not be heated and kept on ice. Trizol protocol:...
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Hello, My understanding is that protein from cell and tissue lysates degrade between each rethaw. Has anyone experienced differing protein concentrations, significant enough to affect loading...
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Most protocols for WBC isolation call for freshly collected blood. How are blood samples kept stable for off-site analysis in which it is shipped overnight? Through freezing? We are trying to...
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I disagree with the thesis of this article from the Physicians Committee for Responsible Medicine, and I am curious to hear thoughts from others....
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Dear Colleagues, Based on clinical and epidemiological data, plant-based diets have emerged as potentially efficacious therapies in the prevention and treatment of various cardiovascular...
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