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Questions related from Rajesh Chaudhary
I have been amplifying telomeres with a real-time PCR experiment and now I am having problems calculating the absolute length of the telomere. From literature reviews I have found that the length...
05 May 2012 5,483 11 View
Can anyone please tell me what is the level of expression of a single copy gene (36B4) compared to telomere in a cell? I mean, all cells might have different level of expression of both genes,...
04 April 2012 290 12 View
Hello friends, I am doing real time pcr for my standard gene which is 36B4. I did serial dilution from 10^-1 to 10^-5. My highest concentration is 200 pg/micro liter and then serial dilution. I...
04 April 2012 9,196 5 View
Dear friends, I am using Agilent Technology company machine and master mix supplied with ROX dye but in seperate vial. When I optimized my reaction using standard curve, I find that I get good...
02 February 2012 1,481 5 View
