Rajdeep Roy

3 Questions 4 Answers 0 Followers

Questions related from Rajdeep Roy

How can I get a prominent band of Phospho-mTOR (289 KD) in western blot ?

Hello everyone, I am performing western blot (SDS-PAGE) for the phospho-mTOR protein, molecular weight 289 KD. I am using 6% resolving gel for it. I kept the transferring time around 4 to 6 hours...

19 January 2024 1,038 1 View

I am not getting protein in the nuclear fraction while performing cytoplasmic and nuclear extraction why?

I am performing cytoplasmic and nuclear extraction from different cell line to check the expression level of a particular protein. I am not getting protein in the nuclear fraction.

18 November 2021 989 4 View

How I should prepare the sample (plant extract) for FTIR?

The plant methanol extract is oily and sticky. Its not giving good result in FTIR analysis. But the extract is fresh.

13 August 2018 4,287 5 View