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Questions related from Rajdeep Chowdhury
I am looking for an IR laser source with wavelength ~800-950 nm to set up a focus lock system. Power should be around ~ 20 mW (more power is also ok). Most importantly, the beam diameter I am...
18 January 2018 1,004 3 View
I use lipofectamine 2000 to transiently transfect my plasmid into the HeLa cells and then use selection antibiotic to make stable cell. This method does not give very good transfection efficiency...
28 July 2017 4,131 1 View
Let's say, I have my x and y data as column vectors. How to make a 2D contour plot from this using Matlab? If anyone has any idea, please suggest me.
09 May 2017 7,500 6 View
I have a plasmid which differs by one base from wild type. This base will change the protein by one amino acid, hence I need to mutate that base which is residing in the central part of the...
07 March 2017 1,944 3 View
When I am transforming a plasmid in BL21 (DE3) cells, plate is found to contain colonies of different size. Roughly they may be classified as small, medium and large. Are they all equivalent for...
26 January 2017 3,012 2 View
I working on the expression of a protein in BL21 cell using IPTG as inducer. Usually after adding the inducer, overnight culture is suggested followed by centrifugation to pallet down the cells. I...
16 January 2017 9,529 7 View
Hey, I am new to molecular biology. I have a plasmid which contains my desired gene and I have amplified the gene using pcr. Now I need to do agarose gel electrophoresis. I have a lambda DNA...
28 November 2016 1,717 7 View
