Rajdeep Chowdhury

5 Questions 4 Answers 0 Followers

Questions related from Rajdeep Chowdhury

What is the most efficient and faster way to generate a stable cell line starting from a plasmid?

I use lipofectamine 2000 to transiently transfect my plasmid into the HeLa cells and then use selection antibiotic to make stable cell. This method does not give very good transfection efficiency...

28 July 2017 4,052 1 View

Is inverse PCR the only option to mutate one central base of a plasmid?

I have a plasmid which differs by one base from wild type. This base will change the protein by one amino acid, hence I need to mutate that base which is residing in the central part of the...

07 March 2017 1,881 3 View

Ideal colony size during plasmid transormation in BL21 (DE3) cells?

When I am transforming a plasmid in BL21 (DE3) cells, plate is found to contain colonies of different size. Roughly they may be classified as small, medium and large. Are they all equivalent for...

26 January 2017 2,954 2 View

Protein expression?

I working on the expression of a protein in BL21 cell using IPTG as inducer. Usually after adding the inducer, overnight culture is suggested followed by centrifugation to pallet down the cells. I...

16 January 2017 9,457 7 View

How to know the number of bps in an unknown DNA supplement in agarose gel and how to record that data?

Hey, I am new to molecular biology. I have a plasmid which contains my desired gene and I have amplified the gene using pcr. Now I need to do agarose gel electrophoresis. I have a lambda DNA...

28 November 2016 1,661 7 View