Rafael Espiritu

4 Questions 9 Answers 0 Followers

Questions related from Rafael Espiritu

Can you recommend a normal human cell line that can undergo necroptosis without the need for transfecting some parts of the necroptosis machinery?

We are interested in doing some comparative studies on necroptosis in human normal and cancer cell lines. We had no problem identifying cancer lines that can undergo necroptosis using e.g., TSZ.,...

22 May 2021 1,481 3 View

Is transient transfection all or nothing?

I performed transient transfection of HeLa cells using Lipofectamine overnight, with a GFP-tagged protein. Seen under a confocal fluorescence microscope, one could clearly see cells with good...

15 March 2020 7,124 4 View

Can I Request for Assistance with sea anemone identification?

May I kindly ask for expert assistance with identifying a sea anemone that was bought from commercial sources in the Philippines. The store staff mentioned that this is the purple tip anemone or...

21 September 2016 1,738 3 View

Can I request assistance to identify the species of fire coral (Millepora sp.)?

I am currently working on secondary metabolites from fire corals (Millepora spp.). Unfortunately, I am having difficulty at getting this particular sample identified to the species level. Checking...

29 February 2016 8,236 10 View