Rachel Sieg

4 Questions 6 Answers 0 Followers

Questions related from Rachel Sieg

Are there any good protocols for Reverse ChIP?

Hi guys, I have done an exhaustive search for protocols for a Reverse ChIP, and I keep running into the same review articles, each citing each other, and I can't find a detailed protocol or...

25 October 2016 2,224 1 View

What is the lowest amount of blood possible to use for Cluster- Chip for capturing freely circulating tumor cells?

Hi! I am trying to determine if using a cluster-chip would be feasible for my project to capture freely circulating tumor cells obtained from an aliquot of blood from Zebrafish. The lowest amount...

24 October 2016 3,593 5 View

Why am I seeing a positive GFP band in my PCR but not under a fluorescent microscope in zebrafish embryos after doing CRISPR/CAS HDR?

I have been working with the Heat Shock Protein 70 (Hsp70) developing a CRISPR/Cas construct to replace Hsp70 with Hsp70-GFP. After microinjecting optically clear zebrafish embryos, I visualized...

22 June 2016 2,070 3 View

When using bodipy tr methyl ester dye as a counter stain against GFP in zebrafish larvae, what is the maximum age that the dye can penetrate?

I am attempting to use the counter stain in fish between 15-25 days post fertilization, and the dye appears to penetrate, but the patterns I am seeing under confocal do not match the organ I am...

01 January 1970 3,848 1 View