Qurshid Hasan Khan

8 Questions 18 Answers 0 Followers

Questions related from Qurshid Hasan Khan

Small RNA Next Generation Sequencing?

I want to do small RNA Next Generation Sequencing. To publish my paper in a high impact journal, do I need to take at least 2 replicates or pooling the sample without any replicate would work. I...

14 December 2017 9,871 2 View

If gap are inserted at the end of the sequence for phylogenetic analysis using MEGA 7.0 software, does it have any impact on tree?

I have been doing phylogenetic analysis for quite sometime utilizing MEGA 7.0 software. The protein sequence which I draw out of database are different lengths. Multiple sequence alignment of...

14 May 2016 2,119 3 View

What does number indicates at nodes in phylogenetic analysis?

Hi I recently started working on phylogenetic analysis. I made a tree the picture is attached below. What does number indicates at nodes of the tree. What more inference can I draw from the...

03 March 2016 10,028 11 View

Double bands after restriction digestion?

I got 2 bands after restriction digestion of unknown gene fragment, does it mean that my gene has restriction site apart from vector. Can I assume that addition of these two band will give me the...

03 January 2016 1,607 4 View

100 a.a. sequence can act as Vacuolar ABC transporter?

Hi I have ABC gene consisting of 3 ORF and I am interested in second ORF coded for vacuolar transporter, which showed 100 a.a. sequence. The aa seq showed signature sequence and walker sequence....

11 August 2015 8,957 3 View

Can someone help with blue-white screening?

I have been doing TA cloning for quite sometime. My sample is giving me only few white colonies. I took two control, the first control is plasmid with insert, which resulted in full plate white...

06 August 2015 1,401 4 View

Can I study leaf fungus protein expression with 2D and MALDI?

I have 2 samples of infected leaves. First sample, I allowed the infection to occur naturally and to second I sprayed fungicide on the leaves and allowed the infection, this would create stress on...

10 June 2015 5,098 3 View

How do I fill overhanging 5' and 3' of double stranded cDNA?

I have 2 cDNA samples to which I have added two different adaptors, adator 1 to 1 cDNA and adator 2 to another cDNA. When I combined the 2 samples of single strand cdna to get double strand cDNA,...

06 May 2015 2,523 6 View