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Questions related from Qurshid Hasan Khan
I want to do small RNA Next Generation Sequencing. To publish my paper in a high impact journal, do I need to take at least 2 replicates or pooling the sample without any replicate would work. I...
14 December 2017 9,829 2 View
Hi I recently started working on phylogenetic analysis. I made a tree the picture is attached below. What does number indicates at nodes of the tree. What more inference can I draw from the...
03 March 2016 9,972 11 View
I got 2 bands after restriction digestion of unknown gene fragment, does it mean that my gene has restriction site apart from vector. Can I assume that addition of these two band will give me the...
03 January 2016 1,551 4 View
Hi I have ABC gene consisting of 3 ORF and I am interested in second ORF coded for vacuolar transporter, which showed 100 a.a. sequence. The aa seq showed signature sequence and walker sequence....
11 August 2015 8,893 3 View
I have been doing TA cloning for quite sometime. My sample is giving me only few white colonies. I took two control, the first control is plasmid with insert, which resulted in full plate white...
06 August 2015 1,367 4 View
I have 2 samples of infected leaves. First sample, I allowed the infection to occur naturally and to second I sprayed fungicide on the leaves and allowed the infection, this would create stress on...
10 June 2015 5,053 3 View
I have 2 cDNA samples to which I have added two different adaptors, adator 1 to 1 cDNA and adator 2 to another cDNA. When I combined the 2 samples of single strand cdna to get double strand cDNA,...
06 May 2015 2,473 6 View
