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In my opinions, there is not cytomegalovirus promoter gene in the human genome, there was not integration in the genome. But there are lots of studied show the stable overexoression by vectors...
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I just want to overexpression a peptides in hepatoma cells is just 15 kDa, but i get the postive protein is almost 60kDa. And the peptide could be binding with a 47kDa protein. I use the thermo...
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We purified a protein with yellowish brown color From Ni-NTA Column, but I don't know why. The protein with 70 amino acid contains 12 cysteines. And I want to seek the protocol that how to...
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Anyone who use XbaI and XhoI enzyme to digest the pet28 for decrease the unnecessary amino acid such as the T7 tag in protein expression. Does it work for protein expression? And why the pet28a...
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We just want to express one hetero-protein. No matter how we change the parameter of different factors, but it is likely not really work. And we also increased the zeocin concentration for...
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