Prospero Civita

6 Questions 9 Answers 0 Followers

Questions related from Prospero Civita

3D spheroids and count?

Hi, I have a question about the count of 3D spheroids. How can I count the number of spheroids starting from the number of seeded cells? After 3 days colture I count spheroids that have 100 um...

27 March 2018 1,717 3 View

Study tumour haeterogenity

Hello,Can anyone suggest a bioinformatic approach to studying tumor heterogeneity in tumor samples? In other words, I performed exome analysis on my samples and now I want to build an evolutionary...

20 July 2017 1,607 1 View

How can evaluate the activity of inverseTrascriptase on a fresh sample?

Hi to all. someone knows or can suggest me a kit to evaluate the activities of inversetrascriptase the cell sample isolated from fresh tissue? Thanks so much

18 November 2016 7,762 3 View

Herceptin 150mg for Infusion is appropriate for Cell Culture?

I would like to test the effect of Trastuzumab (ROCHE) on BT474 cells, but I have only Herceptin for infuison. Is it possible to use it for cell cultures? I have to resusped it in the classic...

26 April 2016 1,950 3 View

How can I confirm the mutation in PMS2 gene obtained through NGS?

Exome data obtained from NGS analysis show the mutation in PMS_2 gene. I would like to confirm the mutation by Sanger sequencing but the PMS2 have a Pseudogene PMS2CL. I'm trying to isolate the...

15 April 2016 4,935 9 View

multivariate Cox regression and SPSS data analysis using gene expression and genetic marker?

Dear all, I am working on gene expression and Kaplan Meier curve dividing the patients in " high" and "low" using SPSS, then I want to do the cox proportional hazard analysis combining i.e the...

01 January 1970 3,359 1 View