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Questions related from Prospero Civita
Dear all, does anyone know which Package on R may I use to retrieve mRNA data from TCGA related to the project GBM 2013? I tried RTCGA package but refers to GBM.rnaseq data which has only 163...
28 June 2020 980 0 View
Hi to all, anyone have stained cell with mitoTracker (invitrogen) and than plate cells for 24h or 48h for post imaging analysis or time-lapse recorder? Thank you for any suggestions?
08 May 2019 3,968 0 View
Hi, I have a question about the count of 3D spheroids. How can I count the number of spheroids starting from the number of seeded cells? After 3 days colture I count spheroids that have 100 um...
27 March 2018 1,740 3 View
Hello,Can anyone suggest a bioinformatic approach to studying tumor heterogeneity in tumor samples? In other words, I performed exome analysis on my samples and now I want to build an evolutionary...
20 July 2017 1,624 1 View
Hi to all. someone knows or can suggest me a kit to evaluate the activities of inversetrascriptase the cell sample isolated from fresh tissue? Thanks so much
18 November 2016 7,774 3 View
Hi, I m sequencing Dna by Genetic Analyzer 31/30 system and i m usiing Applay Biosystem size standart kit. but in my electropherogram after my peak I always found another peak what does it show?
29 April 2016 4,764 4 View
I would like to test the effect of Trastuzumab (ROCHE) on BT474 cells, but I have only Herceptin for infuison. Is it possible to use it for cell cultures? I have to resusped it in the classic...
26 April 2016 1,966 3 View
Exome data obtained from NGS analysis show the mutation in PMS_2 gene. I would like to confirm the mutation by Sanger sequencing but the PMS2 have a Pseudogene PMS2CL. I'm trying to isolate the...
15 April 2016 4,950 9 View
Dear all, I am working on gene expression and Kaplan Meier curve dividing the patients in " high" and "low" using SPSS, then I want to do the cox proportional hazard analysis combining i.e the...
01 January 1970 3,366 1 View
