Prashanti Patel

3 Questions 4 Answers 0 Followers

Questions related from Prashanti Patel

Has anyone used 4 bp overhangs to stitch 5 fragments together into a vector?

I'm trying to clone 5 fragments, each of 200-300 bp in size into a vector and screening with blue-white screening. They each have 4 bp overlapping overhangs. I tried T4 ligase but have been...

03 December 2024 1,532 0 View

Which is the best method for multiple testing correction when doing GO enrichment?

There are several such as the Bonferroni, BH and Benjamini-Yekutieli procedures. However I haven't been able to find literature giving a systematic comparison between these methods. AgriGO gives...

01 June 2020 3,044 0 View

Can anyone suggest preparing CTAB buffer containing PVP K90 for RNA isolation from banana leaves rich in polyphenol and polysaccharide?

This buffer has PVP K90, 2%CTAB, 2M NaCl, Tris EDTA and 4M guanidium thiocyanate. Actually the protocol uses PVPP but I don't have that and can only use PVP-K90. Next is TRIzol isolation but PVP...

02 January 2016 4,401 4 View