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Hello I have been trying to make deletion constructs using site directed mutagenesis. The largest deletion is around 522 basepairs. My plasmid is around 11KB. I have tried inverse PCR primers,...
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Hello I have been using the Agilent quick change lightning kit to generate mutations. I have recently designed a 63 base pair complementary primer (as per the manual) to create 2 mutations...
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Hello, I recently recieved a frozen batch of Dox inducible HeLa cells to generate protein of our intetrest. I have passaged the cells in DMEM with 10% FBS and 1% Penstrep for almost 3 weeks now...
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Hello I am trying to do insertion (1 basepair change, 2 basepair changes) and deletion (50 base deletion) mutations using the Agilent Quikchange Lightning kit. However the mutagenesis does not...
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