4 Questions 7 Answers 0 Followers
Questions related from Pranetha Baskaran
Hello I have been trying to make deletion constructs using site directed mutagenesis. The largest deletion is around 522 basepairs. My plasmid is around 11KB. I have tried inverse PCR primers,...
19 March 2019 4,694 5 View
Hello I have been using the Agilent quick change lightning kit to generate mutations. I have recently designed a 63 base pair complementary primer (as per the manual) to create 2 mutations...
27 November 2017 9,122 3 View
Hello, I recently recieved a frozen batch of Dox inducible HeLa cells to generate protein of our intetrest. I have passaged the cells in DMEM with 10% FBS and 1% Penstrep for almost 3 weeks now...
13 March 2017 6,359 9 View
Hello I am trying to do insertion (1 basepair change, 2 basepair changes) and deletion (50 base deletion) mutations using the Agilent Quikchange Lightning kit. However the mutagenesis does not...
01 January 1970 1,648 8 View