Praneeti Pathipati

4 Questions 12 Answers 0 Followers

Questions related from Praneeti Pathipati

Best way to prep sample for glutathione content and lipid peroxidation assay ?

I'm hoping to use the same (currently frozen brain) sample to measure GSH/GSSG/Total as well as MDA assay - what would be the best way to prep this sample? I'm using the sigma kit for MDA and...

08 January 2018 5,126 6 View

Have you used Creative Bioarray as a supplier?

Hi all, Has anyone used Creative Bioarray (NY) as a supplier for anything you might have needed ? Any cell type in particular? If you have, how reliable did u find them? We're looking for mouse...

18 August 2017 7,372 3 View

Normalizing in Westerns: Anything I can use now after having done all my staining?

I've been having issues with finding a reliable housekeeping protein for normalizing my cytoplasmic & nuclear protein fraction (from H202-injured cells) western blots to. I've checked the...

16 November 2015 8,162 0 View

How to correlate protein expression to cell death on western blots?

I'm looking at the expression of certain proteins in control and injured brain astrocytes and neurons (isolated with MACS). I can't seem to find differences in expression when I know I should at...

08 October 2014 5,455 3 View