Prakit Saingam

5 Questions 6 Answers 0 Followers

Questions related from Prakit Saingam

What is typical practice of quality trimming of sanger sequences?

Hello, I have no experiences with data from sanger sequencing. I amplified 16s rDNA from pure cultures and sent samples for sanger sequencing for identification. Now, I got the data back but...

04 May 2019 5,575 0 View

Is it reasonable to use enriched culture to inoculate MPN?

Hello everyone, I have to quantify pathogen levels in water samples. However, the concentration can be lower than

27 October 2018 6,169 4 View

Why sheep blood agar is used to grow Enterococcus?

Hello, I am curious that why sheep blood agar is recommended as one of the media to grow Enterococcus when some such as E. faecalis would be non-hemolytic? Then, what is the point to supplement...

02 October 2018 9,287 3 View

Can shaking cause fatality of bacteria enriched from soil and sediments?

I regularly have to enumerate bacteria (E. coli and pathogens) from soil and sediment samples. However, I still do not have conclusion for suitable practice. Normally, the enumeration would first...

01 February 2018 4,353 5 View

Has anyone successfully used mCP for the Clostridium perfringens enumeration?

I have problem with the mCP membrane filtration method for Clostridium perfringens. The issue is that when I expose the filter membrane with presumptive Clostridium colonies to ammonium hydroxide,...

16 June 2017 8,580 4 View